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KMID : 0351619710120010159
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Kyungpook Medical Journal
1971 Volume.12 No. 1 p.159 ~ p.173
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An Electron Microcopic Study of the Alterations in the Hepatocyte of Rats with Long-term Administration of DL-ethionine
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Abstract
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The fine structural changes in liver cells injured b}- DL-ethionine have been studied by m,~,ny investigators since Daltpn, in 1:)50.
It has been demonstrated by most authors that DL-ethionine and other c;totosic agents may cause degenerative changes in oraanells of hepatocyte concerned in protein and glycogen metabolism primarily and may secondarily produce various degree and combinations of impairments of specific cellular mechanisms such as synthesis, storage or secretion of protein, glycogen, and lipid. Certain correlations between these cellularmetabolic abnormalities and fine structural alteration have been made, but a great many questions remain unanswered.
Recently Steiner and his coworkers reported a membrane-particle arrays in liver cells fed on ethionine as a speci#ic finding, although the follow-up studies for confirmation remain to be seen.
Ir. an effort to examine further structural change and effects to metabolic abnormalities, the author carried out an experiment with both light and electron microscopical studies on liver
cells of albino rats of Sprague-Dowley strains, weighing between 170 to 230 gm. fed with basal diet (18 o protein) and additiona10.25% D:L-ethionine for 6 and 9 months respectively. Tile following results were obtained:
1. The main alterations in liver cells were fatty changes, necrosis, regenerative ch~.nges, appearance of atypical liver cells and hepatcmalike lesions. Marked proliferation of oval duct epithelial cells and fibrosis (cholangiofibrosis), and cholangioma like lesions were also pronounced. These findings were more pronounced in ~-month-group than in 6-month-group.
2. The tumor like lesions were not observed iu G-month-group while in 9-month-group, the hepatoma-like lesions were noted in 4 out of 24 cases (16.7 ~) and cholangioma like lesions in 10 out of 24 cases (42.7 0) . They were histologically malignant but no evidence of distant metastases.
3. Hepatocytes injured by long term admninistration of ethionine showed various degree and combination of regressive changes in various intracellular organelle of which the main alteration seems to be occurred in organells concerning in protein and glcrcogen metabolism.
4. Increase of lysosomes and the appearance of abnormal structures such as fecal cytoplasmic degradations (FCD) and myelin figures are considered to be related to the phenomena named autophagy or autophagocytosis.
5. It is author´s opinion that the membrane-particle arrays observed by Steiner et. al may not be the specific change in ethionine intoxication in rats.
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KEYWORD
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